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separate his rac2 protein  (Cytoskeleton Inc)


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    Cytoskeleton Inc separate his rac2 protein
    GAP assay using full-length RASAL3 protein. The GTPase activating activity of Rac1 and <t>Rac2</t> was examined in the presence or absence of whole RASAL3 protein, which was prepared using the pFLAG-CMV/hRASAL3 vector. Means ± standard deviation of three independent experiments are represented. The panel within the graph represents an image of western immunoblotting with anti-FLAG antibody (MW was determined as 120 kDa). RASAL3, Ras activating protein-like 3; GAP, GTPase activating protein; MW, molecular weight; WCL, whole cell lysate.
    Separate His Rac2 Protein, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/separate his rac2 protein/product/Cytoskeleton Inc
    Average 94 stars, based on 4 article reviews
    separate his rac2 protein - by Bioz Stars, 2026-03
    94/100 stars

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    1) Product Images from "RASAL3 preferentially stimulates GTP hydrolysis of the Rho family small GTPase Rac2"

    Article Title: RASAL3 preferentially stimulates GTP hydrolysis of the Rho family small GTPase Rac2

    Journal: Biomedical Reports

    doi: 10.3892/br.2018.1119

    GAP assay using full-length RASAL3 protein. The GTPase activating activity of Rac1 and Rac2 was examined in the presence or absence of whole RASAL3 protein, which was prepared using the pFLAG-CMV/hRASAL3 vector. Means ± standard deviation of three independent experiments are represented. The panel within the graph represents an image of western immunoblotting with anti-FLAG antibody (MW was determined as 120 kDa). RASAL3, Ras activating protein-like 3; GAP, GTPase activating protein; MW, molecular weight; WCL, whole cell lysate.
    Figure Legend Snippet: GAP assay using full-length RASAL3 protein. The GTPase activating activity of Rac1 and Rac2 was examined in the presence or absence of whole RASAL3 protein, which was prepared using the pFLAG-CMV/hRASAL3 vector. Means ± standard deviation of three independent experiments are represented. The panel within the graph represents an image of western immunoblotting with anti-FLAG antibody (MW was determined as 120 kDa). RASAL3, Ras activating protein-like 3; GAP, GTPase activating protein; MW, molecular weight; WCL, whole cell lysate.

    Techniques Used: GAP Assay, Activity Assay, Plasmid Preparation, Standard Deviation, Western Blot, Molecular Weight

    In vitro binding assay between RASAL3 GAP domain and Rac2. (A) Purified His-Rac2 proteins (2 µg each) and slurries of GST-RASAL3-GAP beads (50 µl) were mixed with 50 µl 1× GAP assay reaction buffer in the presence of 20 µM GDP, GTP or GTPγS at room temperature for 30 min. The bound proteins were washed and resolved on SDS-PAGE and stained with Coomassie Blue. (B) In vitro bindings between the GST-RASAL3-GAP and each small G-protein were independently performed with 50 mM Tris-Cl (pH 7.5) containing 150 mM NaCl and 20 µM GTP at room temperature for 30 min (upper). The quantities of GTPase pulled down with GST-RASAL3 GAP domain were expressed by relative image density, which was normalized against each GTPase alone (lower). RASAL3, Ras activating protein-like 3; GAP, GTPase activating protein; GST, glutathione S transferase.
    Figure Legend Snippet: In vitro binding assay between RASAL3 GAP domain and Rac2. (A) Purified His-Rac2 proteins (2 µg each) and slurries of GST-RASAL3-GAP beads (50 µl) were mixed with 50 µl 1× GAP assay reaction buffer in the presence of 20 µM GDP, GTP or GTPγS at room temperature for 30 min. The bound proteins were washed and resolved on SDS-PAGE and stained with Coomassie Blue. (B) In vitro bindings between the GST-RASAL3-GAP and each small G-protein were independently performed with 50 mM Tris-Cl (pH 7.5) containing 150 mM NaCl and 20 µM GTP at room temperature for 30 min (upper). The quantities of GTPase pulled down with GST-RASAL3 GAP domain were expressed by relative image density, which was normalized against each GTPase alone (lower). RASAL3, Ras activating protein-like 3; GAP, GTPase activating protein; GST, glutathione S transferase.

    Techniques Used: In Vitro, Binding Assay, Purification, GAP Assay, SDS Page, Staining



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    separate his rac2 protein  (Cytoskeleton Inc)
    94
    Cytoskeleton Inc separate his rac2 protein
    GAP assay using full-length RASAL3 protein. The GTPase activating activity of Rac1 and <t>Rac2</t> was examined in the presence or absence of whole RASAL3 protein, which was prepared using the pFLAG-CMV/hRASAL3 vector. Means ± standard deviation of three independent experiments are represented. The panel within the graph represents an image of western immunoblotting with anti-FLAG antibody (MW was determined as 120 kDa). RASAL3, Ras activating protein-like 3; GAP, GTPase activating protein; MW, molecular weight; WCL, whole cell lysate.
    Separate His Rac2 Protein, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/separate his rac2 protein/product/Cytoskeleton Inc
    Average 94 stars, based on 1 article reviews
    separate his rac2 protein - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier

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    GAP assay using full-length RASAL3 protein. The GTPase activating activity of Rac1 and Rac2 was examined in the presence or absence of whole RASAL3 protein, which was prepared using the pFLAG-CMV/hRASAL3 vector. Means ± standard deviation of three independent experiments are represented. The panel within the graph represents an image of western immunoblotting with anti-FLAG antibody (MW was determined as 120 kDa). RASAL3, Ras activating protein-like 3; GAP, GTPase activating protein; MW, molecular weight; WCL, whole cell lysate.

    Journal: Biomedical Reports

    Article Title: RASAL3 preferentially stimulates GTP hydrolysis of the Rho family small GTPase Rac2

    doi: 10.3892/br.2018.1119

    Figure Lengend Snippet: GAP assay using full-length RASAL3 protein. The GTPase activating activity of Rac1 and Rac2 was examined in the presence or absence of whole RASAL3 protein, which was prepared using the pFLAG-CMV/hRASAL3 vector. Means ± standard deviation of three independent experiments are represented. The panel within the graph represents an image of western immunoblotting with anti-FLAG antibody (MW was determined as 120 kDa). RASAL3, Ras activating protein-like 3; GAP, GTPase activating protein; MW, molecular weight; WCL, whole cell lysate.

    Article Snippet: RhoGAP Assay Biochem kit (cat. no. BK105) containing H-Ras, RhoA, Rac1 and Cdc42, separate His-Rac2 protein (cat. no. RC02) and CytoPhos Reagent were purchased from Cytoskeleton, Inc. (Denver, CO, USA).

    Techniques: GAP Assay, Activity Assay, Plasmid Preparation, Standard Deviation, Western Blot, Molecular Weight

    In vitro binding assay between RASAL3 GAP domain and Rac2. (A) Purified His-Rac2 proteins (2 µg each) and slurries of GST-RASAL3-GAP beads (50 µl) were mixed with 50 µl 1× GAP assay reaction buffer in the presence of 20 µM GDP, GTP or GTPγS at room temperature for 30 min. The bound proteins were washed and resolved on SDS-PAGE and stained with Coomassie Blue. (B) In vitro bindings between the GST-RASAL3-GAP and each small G-protein were independently performed with 50 mM Tris-Cl (pH 7.5) containing 150 mM NaCl and 20 µM GTP at room temperature for 30 min (upper). The quantities of GTPase pulled down with GST-RASAL3 GAP domain were expressed by relative image density, which was normalized against each GTPase alone (lower). RASAL3, Ras activating protein-like 3; GAP, GTPase activating protein; GST, glutathione S transferase.

    Journal: Biomedical Reports

    Article Title: RASAL3 preferentially stimulates GTP hydrolysis of the Rho family small GTPase Rac2

    doi: 10.3892/br.2018.1119

    Figure Lengend Snippet: In vitro binding assay between RASAL3 GAP domain and Rac2. (A) Purified His-Rac2 proteins (2 µg each) and slurries of GST-RASAL3-GAP beads (50 µl) were mixed with 50 µl 1× GAP assay reaction buffer in the presence of 20 µM GDP, GTP or GTPγS at room temperature for 30 min. The bound proteins were washed and resolved on SDS-PAGE and stained with Coomassie Blue. (B) In vitro bindings between the GST-RASAL3-GAP and each small G-protein were independently performed with 50 mM Tris-Cl (pH 7.5) containing 150 mM NaCl and 20 µM GTP at room temperature for 30 min (upper). The quantities of GTPase pulled down with GST-RASAL3 GAP domain were expressed by relative image density, which was normalized against each GTPase alone (lower). RASAL3, Ras activating protein-like 3; GAP, GTPase activating protein; GST, glutathione S transferase.

    Article Snippet: RhoGAP Assay Biochem kit (cat. no. BK105) containing H-Ras, RhoA, Rac1 and Cdc42, separate His-Rac2 protein (cat. no. RC02) and CytoPhos Reagent were purchased from Cytoskeleton, Inc. (Denver, CO, USA).

    Techniques: In Vitro, Binding Assay, Purification, GAP Assay, SDS Page, Staining